Holmes had been listed as probable with an ankle injury. He practiced all week, so it's unclear what happened that landed him on the sideline. A.Q. Shipley will start in his place.
Indianapolis' other inactives: receiver Da'Rick Rogers, linebacker Jerrell Freeman, linebacker Chris Carter, guard Lance Louis, guard Joe Reitz and defensive tackle Arthur Jones. Freeman (hamstring) and Jones (ankle) were ruled out Friday. Reitz had been questionable with an ankle injury.
Jacksonville is without receiver Marqise Lee, cornerback Jeremy Harris, running back Storm Johnson, right tackle Austin Pasztor, guard Tyler Shatley, receiver Tavarres King and tight end Clay Harbor. Lee was ruled out Friday.
Rookie receiver Allen Hurns (ankle) is active for the Jaguars.